Giardia Antigen Rapid Test 

1.INTENDED USE

S&C BIOTECH Giardia Antigen Rapid Test is a common intestinal parasite that affects not only dogs but also cats and humans. It can cause symptoms like diarrhea, vomiting, gas, and abdominal discomfort in dogs. The Giardia Antigen Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Giardia antigen (Giardia Ag) in dog’s feces or vomit specimen.

Assay Time: 5-10 min

Sample: Feces or vomit

2.PRINCIPLE OF THE ASSAY

S&C BIOTECH Giardia Antigen Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is Giardia antigen in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Giardia antigen in the specimen. 

3.KIT COMPONENT

• 10×Foil pouches, each containing one cassette, one 40μLpipette and a desiccant
• 10×Assay buffer tubes
• 10×Cotton swabs
• 1×Product Manual

4.TEST PROCEDURE

• Collect dog’s fresh feces or vomit with the cotton swab from dog’s anus or from the ground.  
• Insert the swab into the provided assay buffer tube. Agitates it to get efficient sample extraction.
• Take out the test device from the foil pouch and place it horizontally.
• Use the 40μLpipette to suck the treated sample extraction from the assay buffer tube and place 3 drops into the sample hole “S” of the test device.

Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

5.INTERPRETATION OF RESULTS

Positive: The presence of both C band and T band, no matter T band is clear or vague.

Negative: Only clear C band appears.

Invalid: No colored band appears in C zone, no matter whether T band appears.

6.STORAGE

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

7.PRECAUTIONS

• All reagents must be at room temperature before running the assay.
• Do not remove test cassette from its pouch until immediately before use.
• Do not use the test beyond its expiration date.
• The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
• All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.

8.LIMITATION

S&C BIOTECH Giardia Antigen Rapid Test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. It is suggested to apply a further confirmative method such as PCR when positive result was observed.