SF003H FIPV Ab
S&C BIOTECH Feline Infectious Peritonitis Virus Ab Test is a test cassette to detect the presence of Feline Infectious Peritonitis Virus Ab (FIPV Ab) in cat’s serum, pleural fluid and ascetic fluid specimen.
Feline Infectious Peritonitis Virus Ab Test
1. INTENDED USE
S&C BIOTECH Feline Infectious Peritonitis Virus Ab Test is a test cassette to detect the presence of Feline Infectious Peritonitis Virus Ab (FIPV Ab) in cat’s serum, pleural fluid and ascetic fluid specimen.
Assay Time: 5 - 10 min
Sample: serum, pleural fluid and ascetic fluid specimen.
2. PRINCIPLE OF THE ASSAY
S&C Biotech Feline Infectious Peritonitis Virus Ab Test is based on sandwich method lateral flow immunochromatographic
assay.
3. KIT COMPONENT
- 10×Foil pouches, each containing one cassette, one 40μLpipette and a desiccant
- 10×Assay buffer tubes
- 10×10μLcapillary dropper
- 10×Centrifugal tubes
- 1×Product Manual
4. SPECIMEN PREPARATION AND STORAGE
1.Specimen should be obtained and treated as below.
- Serum or plasma: collect the whole blood for the patient cat, centrifuge it to get the plasma, or place the whole blood into a tube which contains anticoagulants to get serum.
- Pleural fluid or ascetic fluid: collect the pleural fluid or ascetic fluid from the patient dog. Use them in the assay or store at 2-8℃.
2.All specimen should be tested immediately. If not for testing right now, they should be stored at 2-8℃.
5.TEST PROCEDURE
- Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.
- Take out the test card from the foil pouch and place it horizontally.
- Collect 20μL of the prepared specimen into a vial of assay buffer and mix well. Then drop 3 drops (approx. 120μL) of the diluted sample into the sample hole “S” of the test card.
- Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid .
6. INTERPRETATION OF RESULTS
Positive: The presence of both C band and T band, no matter T band is clear or vague.
Negative: Only clear C band appears.
Invalid: No colored band appears in C zone, no matter whether T band appears.
7. STORAGE
The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.
8. PRECAUTIONS
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- Do not use the test beyond its expiration date.
- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
- All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.
9.LIMITATION
S&C Biotech Feline Infectious Peritonitis Virus Ab Test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. It is suggested to apply a further confirmative method such as PCR when positive result was observed.