Malachite green rapid test strip

1.Brief

This product is used to detect Malachite green residue in tissue sample of aquatic products, the whole test procedure need about 40mins, suitable for all kinds of enterprises and inspection agency.

• Sensitivity

• Components

2. Sample preparation

Tissue sample (shrimp need to remove head and shell, then clean clear, fish need to remove scale then clean.) should store in cold avoid light.

1) Cut tissue sample, remove the fat, use homogenizer to homogenize.

2) Weigh 5g homogenized tissue sample into 50ml centrifuge tube.

3) Add 3ml Extracting agent 1, then add 8 mL acetonitrile and1 bottle Extracting agent 2.

4) Shake violently for 2min, centrifuge at 4000 rpm at room temperature for 5min.

5) Take 4ml supernatant into 5ml centrifuge tube, then add 100 uL Oxidizer, up-and-down for 10 times, blow to dry at 60℃ by Nitrogen or air.

6) Add 0.3ml Redissolving solution to the dry tube, then add 0.5 mL n-hexane. Mix evenly, centrifuge at 4000 rpm at room temperature for 1 min, absorb 150uL down-layer solution, ready to test.

3 Operation procedures

1) Read the manual carefully. Return the test strips and sample into room temperature （20℃~30℃）before use.

2) Open a package and take out the test strip (Use it immediately after opening package).

3) Absorb 150ul sample solution into gold label well, react for 2 mins, then blow and beat until the red material completely dissolved in the well.

4) Put the test card flatly, drop all solution of sample in the Sample collect region.

5) Read the result in 8-10min, it is invalid in other time.

4. Result judge

4.1 Negative: Red T line appears, T line has same color or is darker than C line, it means the residue is lower then 2ppb or has no detection limit.

4.2 Positive: T line is lighter than C line, or T line do not appear, it means the residue is higher than 2ppb, the lighter T line compared with C line, the higher of the residue.

4.3 Invalid: C line did not appear. It means something wrong with the procedure or the test strip is invalid, read the manual again or use new test strips to test again.

5.Precautions

1) Do not touch the central white membrane.

2) Do not use test strips out of expiry date.

3) If need to test standard directly, please use our PBST buffer to prepare.

4) Do not repeat use the dropper to avoid cross pollution.

5) Do not eat any of the reagents.

6) Wear the disposable gloves during testing.

7) The test environment should be ventilation at 20℃-30℃.

6.Specificity

This product has no cross reaction with other drugs.

7.Precisions

Use this product and Malachite LC-MS/MS method to detect 150 samples including 94 negative samples and 56 positive samples, the result show that the compliance rate is 98.0% for the 2 methods.

8.Storage and expiry date

Storage:  Store at 4-30 ℃ in dark, do not frozen.

Expiry date: 12 months; date of production is on box.